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Chroma 激光專(zhuān)用濾光片 FISH濾光片 TIRF濾光片 Raman濾光片

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  • 品　　牌：
  • Chroma
  • 主要規(guī)格：
  • FISH濾光片 TIRF濾光片 Raman濾光片
  • 用　　途：
  • 用于顯微鏡，觀察FISH TIRF Raman現(xiàn)象
    • Chroma激光專(zhuān)用濾光片 FISH濾光片 TIRF濾光片 Raman濾光片是常見(jiàn)的集中濾光片類(lèi)型，屬于對(duì)光學(xué)品質(zhì)要求較高的濾光片。 激光專(zhuān)用濾光片用于激光作為光源的顯微鏡，例如激光掃描共聚焦熒光顯微鏡（LSCM），全內(nèi)反射濾光片（TIRF）用于全內(nèi)反射熒光顯微鏡（TIRFM），拉曼濾光片用于拉曼成像（Raman），全內(nèi)反射熒光片用于熒光原位雜交分析（FISH）。 一、熒光顯微鏡 LSCM顯微鏡 TIRFM顯微鏡 FISH熒光原為雜交法 Raman光譜分析 熒光顯微鏡(Fluorescence microscope) ： 熒光顯微鏡是以紫外線為光源， 用以照射被檢物體， 使之發(fā)出熒光， 然后在顯微鏡下觀察物體的形狀及其所在位置。熒光顯微鏡用于研究細(xì)胞內(nèi)物質(zhì)的吸收、運(yùn)輸、化學(xué)物質(zhì)的分布及定位等。 細(xì)胞中有些物質(zhì)，如葉綠素等，受紫外線照射后可發(fā)熒光；另有一些物質(zhì)本身雖不能發(fā)熒光，但如果用熒光染料或熒光抗體染色后，經(jīng)紫外線照射亦可發(fā)熒光，熒光顯微鏡就是對(duì)這類(lèi)物質(zhì)進(jìn)行定性和定量研究的工具之一。 激光掃描共聚焦熒光顯微鏡(laser scanning confocal microscopy,LSCM) 測(cè)針孔和光源針孔始終聚焦于同一點(diǎn)，使聚焦平面以外被激發(fā)的熒光不能進(jìn)入檢測(cè)針孔。 激光共聚焦的工作原理簡(jiǎn)單表達(dá)就是它采用激光為光源，在傳統(tǒng)熒光顯微鏡成像的基礎(chǔ)上，附加了激光掃描裝置和共軛聚焦裝置，通過(guò)計(jì)算機(jī)控制來(lái)進(jìn)行數(shù)字化圖像采集和處理的系統(tǒng)。 TIRFM 全內(nèi)反射熒光顯微鏡 全內(nèi)角反射熒光顯微鏡（total internal reflection fluorescence microscope，TIRFM），利用光線全反射后在介質(zhì)另一面產(chǎn)生衰逝波的特性，激發(fā)熒光分子以觀察熒光標(biāo)定樣品的極薄區(qū)域，觀測(cè)的動(dòng)態(tài)范圍通常在200 nm以下。因?yàn)榧ぐl(fā)光呈指數(shù)衰減的特性，只有極靠近全反射面的樣本區(qū)域會(huì)產(chǎn)生熒光反射，大大降低了背景光噪聲干擾觀測(cè)標(biāo)的，能幫助研究者獲得高質(zhì)量的成像質(zhì)量和可靠的觀測(cè)數(shù)據(jù)。故此項(xiàng)技術(shù)廣泛應(yīng)用于細(xì)胞表面物質(zhì)的動(dòng)態(tài)觀察。 二、激光專(zhuān)用濾光片 FISH濾光片 FITR濾光片 Raman濾光片 NF濾光片的定義 ND濾光片 中性灰度鏡（neutral density filter）又叫中灰密度鏡，簡(jiǎn)稱(chēng)ND鏡，其作用是過(guò)濾光線。這種濾光作用是非選擇性的，也就是說(shuō)，ND鏡對(duì)各種不同波長(zhǎng)的光線的減少能力是同等的、均勻的，只起到減 弱光線的作用，而對(duì)原物體的顏色不會(huì)產(chǎn)生任何影響，因此可以真實(shí)再現(xiàn)景物的反差。 FISH濾光片 熒光原位雜交技術(shù)（Fluorescence in situ hybridization, FISH）是根據(jù)已知微生物不同分類(lèi)級(jí)別上種群特異的DNA序列，以利用熒光標(biāo)記的特異寡聚核苷酸片段作為探針，與環(huán)境基因組中DNA分子雜交，檢測(cè)該特異微生物種群的存在與豐度。 NF濾光片 陷波濾光片，通常也被稱(chēng)作帶阻或者帶抑制濾光片。它可以透過(guò)絕大多數(shù)波長(zhǎng)，但是將特定波長(zhǎng)范圍內(nèi)（阻帶）的光衰減到非常低的水平。拉曼光譜用。 Chroma激光專(zhuān)用濾光片 FISH濾光片 FITR濾光片 Raman濾光片的型號(hào)參數(shù) 熒光濾光片型號(hào) 濾光片類(lèi)型 濾光片說(shuō)明 注釋?zhuān)ㄈ剂希L(zhǎng)通或短通） Blue-27M AS 27mm diameter, mounted in 1.25" threaded ring Ha/8-27M AS 27mm diameter, mounted in 1.25" threaded ring Lum-27M AS 27mm diameter, mounted in 1.25" threaded ring Red-27M AS 27mm diameter, mounted in 1.25" threaded ring Green-27M AS 27mm diameter, mounted in 1.25" threaded ring Ha/8-31 AS 31mm diameter, unmounted Red-31 AS 31mm diameter, unmounted Blue-31 AS 31mm diameter, unmounted Green-31 AS 31mm diameter, unmounted Lum-31 AS 31mm diameter, unmounted Lum-36 AS 36mm diameter, unmounted Green-36 AS 36mm diameter, unmounted Blue-36 AS 36mm diameter, unmounted Red-36 AS 36mm diameter, unmounted Ha/8-36 AS 36mm diameter, unmounted Red-50 AS 50mm diameter, unmounted Lum-50 AS 50mm diameter, unmounted Ha/8-50 AS 50mm diameter, unmounted Green-50 AS 50mm diameter, unmounted Green-50S AS 50mm x 50mm, square Blue-50S AS 50mm x 50mm, square Ha/8-50S AS 50mm x 50mm, square Lum-50S AS 50mm x 50mm, square Red-50S AS 50mm x 50mm, square Ha/8-65S AS 65mm x 65mm, square Red-65S AS 65mm x 65mm, square Lum-65S AS 65mm x 65mm, square Blue-65S AS 65mm x 65mm, square Green-65S AS 65mm x 65mm, square Green-27M AS 27mm diameter, mounted in 1.25" threaded ring Red-27M AS 27mm diameter, mounted in 1.25" threaded ring Lum-27M AS 27mm diameter, mounted in 1.25" threaded ring Blue-27M AS 27mm diameter, mounted in 1.25" threaded ring Lum-31 AS 31mm diameter, unmounted Green-31 AS 31mm diameter, unmounted Blue-31 AS 31mm diameter, unmounted Red-31 AS 31mm diameter, unmounted Red-36 AS 36mm diameter, unmounted Blue-36 AS 36mm diameter, unmounted Lum-36 AS 36mm diameter, unmounted Green-36 AS 36mm diameter, unmounted Green-50 AS 50mm diameter, unmounted Lum-50 AS 50mm diameter, unmounted Red-50 AS 50mm diameter, unmounted Red-50S AS 50mm x 50mm, square Lum-50S AS 50mm x 50mm, square Green-50S AS 50mm x 50mm, square Blue-50S AS 50mm x 50mm, square Green-65S AS 65mm x 65mm, square Blue-65S AS 65mm x 65mm, square Red-65S AS 65mm x 65mm, square Lum-65S AS 65mm x 65mm, square HQ493/16x EX Spectrum Green? 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