丙型肝炎病毒NS3(解旋酶)蛋白片段 |
Catalog Number: HCV-NS3h 產(chǎn)品目錄號:HCV-NS3h |
Genotype: 2a 基因型:2a |
Form: Purified via Nickel affinity chromatography 形式:鎳柱親和層析純化 |
Tag: Expressed with a C-terminal 6X His-tag 標簽:C端6X His-tag表達 |
Origin: E.coli 來源:E.coli |
Applications: ELISA、Flow Cytometry、SDS-PAGE、Western blot 應(yīng)用:酶聯(lián)免疫吸附實驗(ELISA)、流式細胞分析(Flow Cytometry)、SDS-PAGE、免疫印跡(Western blot) |
Buffer/Composition: 1X PBS pH 7.2; 1X protease inhibitor cocktail, 1 mM PMSF 緩沖液/成分:1X PBS pH 7.2;1X protease inhibitor cocktail;1 mM PMSF |
Volume: 50μl/ 250μl/ 500μl 體積:50μl/ 250μl/ 500μl |
Concentration: 2 μg/μl 濃度:2 μg/μl |
Storage: Short Term: 4℃ Long Term (up to 1 year): -20℃(avoid repeated freeze/thaw) 儲存:短期:4℃ 長期(可達1年):-20℃(避免反復凍融) |
Coomassie stained gel showing purification of recombinant NS3 helicase protein.Bacterial cells expressing the His-tagged protein were lysed (lane 2), pelleted, and the SN (lane 3) was loaded onto Nickel resin. The ~55 kD protein was absent in the resin flow thru (lane 4) as well as column washes (lanes 5 and 6). Elutions with immidazole (lanes 7 and 8) yielded ≥95% pure protein.Lane 1 = marker
考染顯示純化的重組NH3解旋酶蛋白。表達His標簽蛋白的菌細胞裂解物(lane 2),顆粒狀的SN(lane 3)過鎳樹脂。~55 kD蛋白在樹脂流出液((lane 4)中消失,同見于柱洗滌液(lanes 5 & 6)。洗脫液連同咪唑(lanes 7 & 8)產(chǎn)生≥95%純度的蛋白質(zhì)。Lane 1 = marker
Item #: HCV-NS3h & HCV-NS3h-500 & HCV-NS3h-1
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